Ocean Optics Linear Variable Filters Reshape Traditional Fluorescence Spectroscopy Techniques
Novel LVF-HL and LVF-UV-HL Filters Replace Expensive Filters and Spectrally Shape Broadband Excitation Energy
Dunedin, FL -- Ocean Optics patented LVF-HL and LVF-UV-HL Linear Variable Filters are changing the way that scientists approach traditional fluorescence spectroscopy measurements by eliminating the need for multiple, expensive bandpass filters and by spectrally shaping the excitation energy from a single broadband source. A single LVF cassette can replace multiple filters that often cost more than $100 each. Additionally, LVF sets enable the user to shape the excitation energy from a single broadband source as opposed to using multiple light sources with various wavelengths or a front-end scanning monochromator.
The LVF-HL (300-750 nm) and the LVF-UV-HL (230-500 nm) are high-pass and low-pass filters fastened together in a single filter cassette that can be inserted easily into a fluorescence setup using a variety of LVF accessories. Each LVF cassette features an adjustable center wavelength and an adjustable bandpass across the filter's effective range. The filters are pre-set with a transmission bandwidth at 25 nm, but easy-to-adjust screws allow the user to create a transmission bandwidth as wide as 100 nm or as narrow as 20 nm by positioning the filters against one another.
Additionally, LVF slides can be used on the emission side of a fluorescence experiment to better discriminate between a fluorophore's weak emission wavelengths and those of a more powerful excitation source.
The LVF-HL and LVF-UV-HL feature excellent transmission bands at ~90% and blocking bands ~99.8%. The quartz filters, coated with an interference pattern, are only 57 mm x 10 mm.
In addition to the LVF-HL and LVF-UV-HL, Ocean Optics offers a variety of patented Linear Variable Filters including single high- and low-pass filters, and double high- and low-pass filters that increase the blocking band to 99.96%.
Source: Ocean Optics, Inc.